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Salivary glycoproteins of bloodsucking arthropods
Sumová, Petra ; Volf, Petr (advisor) ; Mikeš, Libor (referee)
During obtaining their blood meal, bloodsucking arthropods salivate into their host. Bloodsucking arthropods' saliva contains wide array of bioactive macromolecules. Host organism develops antibody response against many of these molecules. Due to interspecies variability in salivary protein composition, detection of antibody response may serve as a marker of the exposure to individual species of bloodsucking arthropods. Host antibody response is mostly elicited by proteins or glycoproteins. Glycoproteins contain one or more oligosaccharide chains attached to the protein. Glycoprotein's antigenicity could be caused by either both parts, or by only the protein, or the sugar part. This fact has to be taken into consideration for choice of the expression system for recombinant glycoprotein synthesis. This work summarizes current knowledge about structure, function and features of salivary glycoproteins in various species of bloodsucking arthropods.
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Alternative vectors of Leishmania (Kinetoplastida: Trypanosomatidae)
Jursová, Klára ; Sádlová, Jovana (advisor) ; Dvořák, Vít (referee)
The life cycle of Leishmania (Kinetoplastida: Tripanosomatidae) alternates between vertebrate hosts and insect vectors. Phlebotomine sand flies (Diptera: Psychodidae) were supposed to be the only arthropod vectors supporting morphological and functional development of the parasite including production of infective metacyclic forms capable to infect the vertebrate host. Recently also other bloodsucking arthropods have been suggested for possible transmission of some Leishmania species. The bachelor thesis summarizes the recent knowledge about these alternative vectors of Leishmania.
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Identification of bloodmeal sources in phlebotomine sand flies and other bloodfeeding arthropods
Kykalová, Barbora ; Dvořák, Vít (advisor) ; Spitzová, Tatiana (referee)
Hematophagous arthropods that are involved in transmission of many infectious diseases have profound importance in human and veterinary medicine. Bloodmeal identification considerably contributes towards better understanding of vector-borne diseases and vector-host interaction. It reveals epidemiologically significant data on reservoir hosts and degree of anthropophily of studied arthropods. Methods of bloodmeal identification evolved from field observations, serological and DNA-based methods towards protein analyses. Nowadays, the most frequent methods are DNA-based methods but new methods are still being developed. This bachelor thesis summarizes and compares used methods with the primary focus on phlebotomine sand flies, which are involved in transmission of leishmaniases. Key words: sand flies, bloodmeal identification, hematophagous arthropods, analysis
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Salivary glycoproteins of bloodsucking arthropods
Sumová, Petra ; Volf, Petr (advisor) ; Mikeš, Libor (referee)
During obtaining their blood meal, bloodsucking arthropods salivate into their host. Bloodsucking arthropods' saliva contains wide array of bioactive macromolecules. Host organism develops antibody response against many of these molecules. Due to interspecies variability in salivary protein composition, detection of antibody response may serve as a marker of the exposure to individual species of bloodsucking arthropods. Host antibody response is mostly elicited by proteins or glycoproteins. Glycoproteins contain one or more oligosaccharide chains attached to the protein. Glycoprotein's antigenicity could be caused by either both parts, or by only the protein, or the sugar part. This fact has to be taken into consideration for choice of the expression system for recombinant glycoprotein synthesis. This work summarizes current knowledge about structure, function and features of salivary glycoproteins in various species of bloodsucking arthropods.
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